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. 2021 Jan 21;22(3):1040. doi: 10.3390/ijms22031040

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of 4′,6-diamidino-2-phenylindole (DAPI) concentrations on polyphosphate detection in platelets in 0.1% Tween-20-containing Hanks balanced salt solution (Tw-HBSS). Platelets immobilized on glass slides using a Cytospin were fixed in 10% neutral-buffered formalin for 4 h and then treated with DAPI at (a) 2.5, (b) 5.0, (c) 10, or (d) 20 μg/mL (green) and phalloidin (red) in Tw-HBSS and examined using a fluorescence microscope. (e) Quantitative analysis of the fluorescence images. Significant differences were obtained by a parametric test but not by a non-parametric test.