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SEC-HPLC assessment: (A) Apo-proteins rhHPX and HPX; (B) HPX/Heme samples at an L/P of 0.5 and 2.0 vs. HPX; (C) Heme complexes with rhHPX (L/P of 0.5 and 2.0) vs. rhHPX and PEG-HPX. Detection of the peaks was performed at 280 nm and 413 nm as specified on the plots.
