Figure 5.
Effects of overexpressing PtoCYCD3;3 on Populus stem development. (A,B,D) Cross-sections of the second, 10th, 20th internode in stems of wild-type (WT) and 35S::PtoCYCD3;3 plants. (C,E) Cambial-derived tissue of the 10th and 20th internode in stems of WT and 35S::PtoCYCD3;3 plants. (F) Width of cambial-derived tissue, secondary phloem, and secondary xylem of the 10th internode in stems of WT and 35S::PtoCYCD3;3 plants. (G) Width of cambial-derived tissue, secondary phloem, and secondary xylem of the 20th internode in stems of WT and 35S::PtoCYCD3;3 plants. (H) Vessel area of the 10th and 20th internode in stems of WT and 35S::PtoCYCD3;3 plants. Co: cortex, P: phloem C: cambial, X: xylem, CD: cambial-derived tissue. Significance as determined by independent sample T-test: * p < 0.05, ** p < 0.01. The values are means ±SE, n = 3. Three transgenic lines were used. Bar, 100 µm (A); 200 µm (B–E).