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. 2021 Jan 21;26(3):548. doi: 10.3390/molecules26030548

Figure 4.

Figure 4

Effect of intrathecal injection of NAN-190 or ketanserin on Z. officinale-dependent inhibition of oxaliplatin-induced neuropathic pain in mice (a,b). Spinal mRNA expression level of 5-HT1A receptors before and after oxaliplatin and Z. officinale treatment (c). Cold (a) and mechanical (b) allodynia were assessed by using the acetone drop and von Frey filament tests, respectively. On day 3 following oxaliplatin injection, NAN-190 or ketanserin was injected intrathecally in mice. PBS was used as a control for both NAN-190 and ketanserin. The relative mRNA expression of 5-HT1A was measured before and after oxaliplatin injection and Z. officinale treatment (c). Control (Oxa + PBS + DW): n = 6, Oxa + PBS + Z. officinale: n = 6, Oxa + NAN-190 + DW: n = 5, Oxa + Ketanserin + DW: n = 5, Oxa + NAN-190 + Z. officinale: n = 7, Oxa + Ketanserin + Z. officinale: n = 7. ns: non-significant, ** p < 0.01, *** p < 0.001, **** p < 0.0001 vs. Control (Oxa + PBS + DW) with two-way ANOVA followed by Tukey’s post-test for multiple comparisons (a,b). Control: n = 6, Oxa: n = 8, Oxa + Z. officinale: n = 6. * p < 0.05 vs. Control, ## p < 0.01 vs. Oxa with one-way ANOVA followed by Tukey’s post-test for multiple comparisons (c).