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. 2021 Jan 21;22(3):1049. doi: 10.3390/ijms22031049

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effects of in vitro and ex vivo simulated digestion on rTBL-1. Simultaneous gastric and intestinal in vitro digestion of rTBL-1 was carried out, and the protein was determined at 30 and 60 min (280 nm). (A) Bioaccessibility. (B) Apparent permeability coefficient (P_app) and efflux ratio (ER) during the intestinal incubation. The results are expressed as the mean ± SD of three independent experiments in triplicates. The asterisks express significant differences (p ≤ 0.05) by t-student test for each pair of data between 30 and 60 min. AP, apical side; BL, basolateral side; GC, gastric content; NDF, non-digestible fraction; DF, digestible fraction; P_app, apparent permeability coefficient.