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NRH induced 4NADO production in HepG3 cells but not in HEK293T. HEK293T (A) and HepG3 (B) cells were continuously dosed with 100 µM of 4PYR for 1, 4, and 24 h. The crude cell extract was measured by LC-MS for its NADO content. The NADO detected matches the 4NADO standard. Results are presented as relative abundance to control ± SEM of three independent experiments. Statistical significance: **** p < 0.0001. N.D: Not Detected.
