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. 2021 Jan 27;13(3):489. doi: 10.3390/cancers13030489

Figure 2.

Figure 2

HPV16 E7 disrupts DREAM by displacing LIN52. (A). IP/WB assay shows interaction between p130 and E7 in N/Tert-1+HPV16 cell lines. (B). IP/WB analysis of DREAM and MMB complexes in N/Tert-1 cell lines. Specific bands are indicated by lines. (C). Protein sequence of LIN52 aligned with E7 LXCXE motif. Arrowhead shows residue mutated in LIN52-S20C; pS is the DYRK1A phosphorylation site. (D). IP/WB assay analysis of DREAM complex in the indicated cell lines. (E). Expression of LIN52 alleles detected using IP and WB with anti-HA antibodies. (F,G). Cell proliferation assay with indicated cell lines. * p < 0.05 (t-test) indicates significant difference with the control cell line.