Figure 4.
The inhibition of PKC enhances the internalization of KV1.5–KVβ1.3 channels. Representative confocal images of HEK293 cells transfected with KV1.5–KVβ1.3 under control conditions and after treatment with calphostin C. Fluorescence profiles and fluorescence correlation of internalized channels and plasma membrane ones (bottom) in control and after calphostin-C treatment. Note that control cells show mostly a high red signal (plasma membrane channels), whereas those treated with calphostin C present a weaker red signal, but a much greater green signal (internalized channels, which did not recycle to the cell membrane).