Figure 3.

C21 did not affect the total number of microglia or astrocytes at the ischemic hemisphere. (A) Stitch illustration from a sham animal used to highlight the regions of interests (ROIs) in an intact brain section. Data are the average count of the 3 different ROIs in the ipsilateral RO1, 2 and 3 for GFAP+ and Iba1+ cells. Regions of interest ROI 1 and ROI 3 correspond to the neocortex, whereas ROI 2 corresponds to the corpus callosum and associated subcortical white matter. (B) Representative of stroked vehicle-treated animal taken from the cortex (ROI 1). (C) Representative of stroked C21-treated animal taken from the cortex (ROI 1) as well. C21 administration did not impact the number of GFAP+ (D) or Iba1+ (E) cells (n = 5–6/group). No significant differences were observed in individual ROs or the average ROIs across groups.