Fig. 8.

Structural visualization and examples of paradigmatic mutations selected from the study. a General view of the adenosine A2A receptor (PDBid: 4EIY) as a prototype GPCR with the TM boundaries indicated in light yellow and ECLs/ICLs regions in blue. The ligand-binding cavity is indicated by a solid red surface and G protein-interacting site by a blue arrow. Selected topological positions C^45.50, D^2.50, R^3.50, and P^7.50 are highlighted in the structure as green vdW spheres. b–d Closer look of the atomic environment of selected positions (green sticks) and surrounding residues (salmon). e Sequence conservation logos around the selected positions (number corresponds to their conservation percentage in the MSA). All the residue positions are referenced following the BW convention