Table 1.
Summary of different STDP paradigms induced t-LTP at the hippocampal SC-CA1 synapses in 6-month-old C57Bl/6J and APP/PS1 mice.
| STDP Paradigm (Presyn. Stim.: Postsyn. AP) | No. of Repeats, Frequency | Normalized t-LTP Magnitude (EPSP Slope %) | ||
|---|---|---|---|---|
| C57Bl/6J Mice | WT Littermates | APP/PS1 Mice | ||
| 0:0 (negative control) | 106.9 ± 7.7% (n = 11/N = 10) | |||
| 1 EPSP:4 AP | 6×, 0.5 Hz | 157.9 ± 14.2% (n = 11/N = 8) *,# | 153.8 ± 13.5% (n = 13/N = 7) * | 138.9 ± 8.7% (n = 12/N = 6) * |
| 1 EPSP:1 AP | 6×, 0.5 Hz | 134.3 ± 7.5% (n = 13/N = 10) * | ||
| 1 EPSP:4 AP | 35×, 2 Hz | 139.1 ± 9.5% (n = 10/N = 10) *# | ||
| 1 EPSP:1 AP | 100×, 2 Hz | 142.0 ± 7.9% (n = 13/N = 12) *# | 130.9 ± 8.5% (n = 11/N = 5) * | 157.3 ± 14.9% (n = 9/N = 5) * |
| Aβ Plaque Staining (Methoxy-X04) | ||||
| WT littermates | APP/PS1, cells near to Aβ (<200 µm) | APP/PS1, cells distant from Aβ (>200 µm) | ||
| 1 EPSP:4 AP | 6×, 0.5 Hz | 135.8 ± 10.6% (n = 16/N = 9) * | 92.4 ± 9.1% (n = 12/N = 9) # | 129.6 ± 9.6% (n = 8/N = 4) * |
| Chronic Fingolimod Treatment of APP/PS1 Mice | ||||
| WT littermates + fingolimod | AD near + Fingolimod | |||
| 1 EPSP:4 AP | 6×, 0.5 Hz | 169.9 ± 20.1% (n = 12/N = 7) * | 111.1 ± 10.2% (n = 16/N = 7) | |
n: number of experiments/slices, N: number of animals, * p < 0.05, one-sample t-test (significant t-LTP compared to its own baseline); # p < 0.05, ANOVA 6× 1:4 vs. 0:0 WT; 35× 1:4 vs. 0:0 WT, 100× 1:1 vs. 0:0 WT; APP/PS1 near Aβ vs. WT littermates.