FIGURE 4.

GLZ treatment reduces ACE‐2 expression in the bronchial tissue of weanling piglets. (a) Representative IHC stains of ACE‐2 expression in the bronchial tissue of weanling piglets with sham, TAB, and GLZ treatments at the 10th week of age. In the bronchial tissue, IHC expression of ACE‐2 (indicated with dark brown color) in weanling piglets with GLZ treatment was lower than that in those with sham and TAB treatments; scale bar = 30 μm. (b) Using Western blotting, ACE‐2 expression in the bronchial tissue of weanling piglets with sham, TAB, and GLZ treatments at the 10th week of age. In the bronchial tissue, quantified expression of ACE‐2 in weanling piglets with GLZ treatment was significantly lower than that in those with sham and TAB treatments. All data are shown as mean ± SEM (**p < .01, one‐way ANOVA followed by Student–Newman–Keuls multiple comparison posttest). Experiments were repeated thrice for each treatment