Fig. 1.

Ube3a-del mice exhibited seizure susceptibility and increased spiking compared to WT during baseline EEG recordings. Seizure susceptibility measures were observed for 30 min before and after an i.p. injection of 80 mg/kg PTZ. a Reduced latencies to first jerk were observed in the Ube3a-del mice compared to WT. b Faster onset to generalized tonic–clonic seizure was observed in Ube3a-del versus WT. To assess hyperexcitability, c mice were implanted with a telemetric device that collected both EEG and EMG data and d was small enough to allow for untethered, unrestrained data collection from the home cage of the test animals. e, f Representative EEG traces of both WT and Ube3a-del animals sampled before convulsant administration. Quantification of spiking activity during baseline data acquisition in mice recorded for g 72 h and h 24 h indicated more spiking events in Ube3a-del animals compared to their WT littermate controls. *p < 0.05, Student’s t test between genotype