Fig. 5.

Inhibition of S100A9 mitigates the LPS-induced cytokine secretion via the NLRP3 pathway in vitro. a, b The NLRP3 overexpression efficiency was evaluated by western blot analysis at 24 h following transfection. c-, d S100A9 blockade attenuated the secretion of TNF-α and IL-1β via the NLRP3 pathway. e, f The mRNA expression levels of TNF-α and IL-1β were determined by RT-PCR. Data are expressed as the mean ± SEM (n = 4–6 for each group). Statistical analysis was carried out using one-way ANOVA followed by Tukey's multiple comparison test. S100A9 S100 calcium binding protein A9, LPS lipopolysaccharide, NLRP3 NOD-like receptor family pyrin domain containing 3, TNF-α tumor necrosis factor α, IL-1β interleukin 1β