Fig. 6.

S100A9 blockade exerts its protective effects via inhibiting the NLRP3 pathway. a Co-immunoprecipitation was performed to detect the interaction of S100A9 with its receptor (TLR4) in the lung tissues of LPS-treated mice. b The protein expression levels of NF-κB and apoptosis-related proteins were measured by western blot analysis. c, d The quantified results are shown. e The lung injury score was determined based on histological analysis. f The lung wet-to-dry weight ratio is presented. g The total protein levels in BALF are shown. h, i The MPO activity and LDH levels in the lungs of mice are shown. Data are expressed as the mean ± SEM (n = 6 for each group). Statistical analysis was performed using two-way ANOVA followed by Tukey's multiple comparison test. ^*P < 0.05, ^**P < 0.01 vs. the control group. S100A9 S100 calcium binding protein A9, NLRP3 NOD-like receptor family pyrin domain containing 3, TLR4 toll-like receptor 4, LPS lipopolysaccharide, NF-κB nuclear factor κB, BALF bronchoalveolar lavage fluids, MPO myeloperoxidase, LDH lactate dehydrogenase