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. 2021 Feb 6;23:52. doi: 10.1186/s13075-021-02434-w

Fig. 4.

Fig. 4

IKKβ-mediated A20 phosphorylation of the TNFAIP3 variants. Immunoblot of lysates from A20-deficient HEK293 cells transfected with the vectors encoding A20 WT or variants, with or without co-transfected IKKβ, and probed with antibodies to p-S381 A20, myc (detecting A20), FLAG (detecting IKKβ), and β-actin. a The loss of IKKβ-mediated S381 phosphorylation was observed with the known mutant C243Y. b None of the three variants (E192K, I310T, and Q709R) caused reductions in IKKβ-mediated phosphorylation of A20 S381