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. 2021 Feb 5;12:113. doi: 10.1186/s13287-021-02171-6

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — a Histogram (FACS) showing BDL-E5⁺ cell populations at 7 dpn: DPSC1 (i) and DPSC2 (ii). Relative cell count is indicated on the y-axis, and fluorescence intensity (Texas Red channel) on the x-axis. The top 10% and bottom 10% of cell populations are as indicated (n = 3). b (i) Fluorescent images of BDL-E5, TRA-1-60, and transmitted light (TL) images (4X) showing iPS colonies derived from BDL-E5⁺ and BDL-E5⁻ cell populations of DPSC1 and DPSC2 following FACS at 7 dpn. (ii) Graph showing average number of DiPS colonies in reprogrammed BDL-E5⁺ and BDL-E5⁻ cell populations obtained after FACS of DPSC1 and DPSC2. **p < 0.01 and ***p < 0.001 denote statistical significance (n = 3). c Histogram showing FACS of BDL-E5⁺ and BDL-E5⁻ cell populations at 14 dpn from SC-ASC S15 (i) and VS-ASC S15 (ii). The percentage of positively and negatively stained cells is shown. (iii) Graph showing average number of iPS colonies in BDL-E5⁺ and BDL-E5⁻ cell populations obtained after FACS at 14 dpn from SC-ASC S15 and VS-ASC S15. *p < 0.05 denotes statistical significance. (iv) TL images (10X) showing iPS colonies from SC-ASC S15 and VS-ASC S15 following FACS for BDL-E5⁺ and BDL-E5⁻ cell populations. Similar results were obtained with S16-derived SC-ASC and VS-ASC (n = 3). Scale bar represents 100 μm