Table 10.
Primers, amplification conditions, and PCR-RFLP for SNPs.
| SNP | Primer Sequence 5′–3′ | Annealing (°C) | Product Size (bp) | Restriction Enzyme | Digestion Fragments (bp) |
|---|---|---|---|---|---|
| IL-6 −174 G > C rs 1800795 |
For: ATGCCAAGTGCTGAGTCACTA Rev: TCGAGGGCAGAATGAGCCTC |
58.5 | 230 |
Nla-III
(NEB®) |
GG: 230 GC: 230, 121/109 CC: 121/109 |
| IL-8 −251 T > A rs4073 |
For: TCATCCATGATCTTGTTCTAA Rev: GGAAACGCTGAGGTCGA |
57 | 542 |
Mfe-I
(NEB®) |
TT: 542 TA: 542, 450, 92 AA: 450, 92 |
| IL -8 +781 C/T rs2227306 |
For: CTCAACTCTTTATATAGGAATT Rev: GATTGATTTTATCAACAGGCA |
54 | 203 |
EcoRI
(NEB®) |
CC: 184, 19 CT: 203, 184, 19 TT: 203 |
bp = base pairs.; For: Forward; Rev: Reverse