Figure 3.

Blocking FFaR2 prevents BA-mediated collagen type I and p-ERK induction. 3T3 cells were transfected with FFaR2 (siFFaR2) or scrambled siRNA followed by treatment with and without BA for 24 h. 3T3 cells treated with H₂O were included as a control. (a) Protein expression analyzed by the Western blot analysis of collagen type I and the ratio of intensities of collagen type I to β-actin. (b) The expression of the FFaR2 gene relative to the GAPDH gene by RT-PCR analysis. (c) Protein expression analyzed by the Western blot analysis of p-ERK1/2, ERK1/2, and β-actin and the ratio of intensities of pERK1/2 and ERK/2 relative to β-actin and the ratio of intensity of p-ERK1/2 to ERK1/2 in the above groups are displayed. Data are expressed as means ± SD (*** p < 0.001; n = 3).