Skip to main content
. 2021 Feb 6;24(3):102151. doi: 10.1016/j.isci.2021.102151

Figure 7.

Figure 7

HSP90 inhibitors treatment reduce SARS-CoV-2 replication and induction of pro-inflammatory cytokines

(A) Fold change of infectious plaque-forming units (PFUs) in supernatants of Calu-3 cells infected with SARS-CoV-2 with the indicated treatment 16 hpi. After viral adsorption for one hour, cells were washed and supplied with conditioned medium containing DMSO, as solvent control, or indicated concentrations of Onalespib, Ganetespib, or 17-AAG. Experiment performed in triplicates, and error bars represent standard deviations.

(B) Percentage of viral sequencing reads of all reads in Calu-3 cells after 800 nM of indicated inhibitor treatment 16 hpi.

(C) Heatmap of selected induced genes normalized to infection in Calu-3 cells without treatment.

(D) Infectious viral particles in the supernatant at the indicated timepoints post infections of primary human airway epithelial cells (AECs) treated with either DMSO (control, black) or 200 nM 17-AAG (blue).

(E) Heatmap of selected induced genes in AECs normalized to the infection sample treated with DMSO of the same timepoints (average of normalized expression values/FPKM).