Figure 2.

IGF-1 increased the protein levels of biglycan and decorin by upregulating protein translation in NHDFs. (a) Primary NHDFs were treated with actinomycin D (Act D, 1 µg/mL) or cycloheximide (CHX, 25 µg/mL) under basal conditions or in the presence of IGF-1 (250 ng/mL) for 18 h. Proteins of conditioned media and cell lysates were analyzed by western blotting. (b) Relative protein amounts were analyzed using ImageJ. Band intensity was normalized to that of β-tubulin. Values represent the mean ± SEM of data (N = 4). Statistical comparison was made using paired t-test. (* p < 0.05, ** p < 0.01).