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Immunofluorescence analyses of Cx43 expression in transfected HeLa cells. Cadherin was used to stain cell membranes. Primary antibodies (1:1000): antipan cadherin (mouse), anti Cx43 (rabbit); secondary antibodies (1:500): Alexa 488 (antimouse), Alexa 594 (antirabbit). DAPI (1:10,000) was used to stain cell nuclei.
