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. 2021 Jan 31;22(3):1417. doi: 10.3390/ijms22031417

Figure 4.

Figure 4

Assessment of adenosine receptor-mediated cAMP production in A2AAR-transfected (red) and nontransfected (green) HeLa- cells. The cAMP-activated GloSensor luciferase was cotransfected resulting in cAMP-dependent luminescence signals. Cells (60,000/well) were incubated with medium supplemented with 2% GloSensor luciferase reagent for 2 h at 37 °C. After addition of DMSO or compound dissolved in DMSO (forskolin, 10 µM, CGS-21680, 100 µM, or NECA, 50 µM) the cells were incubated at 37 °C for 15 min. Signals induced by adenosine receptor agonists CGS-21680 or NECA were significantly different between both cell lines (*** p < 0.001, 2-way ANOVA). For details see Section 4.