Figure 6.

cAMP-dependent luminescence signal due to activation of cocultures of donor and biosensor cells (3:1) by the selective A_2AAR agonist CGS-21680 (1 µM). Forskolin (10 µM) was used as positive control and DMSO (1%) served as negative control. Data are normalized to the maximal effect of forskolin (100%). Data represent means ± SEM of three individual experiments performed in duplicates. (a) Activation without PDE inhibitor. Only forskolin displayed a significantly increased luminescence signal compared to control. (b) Activation in the presence of the PDE inhibitor IBMX (200 µM). Statistical significance calculated with repeated measures 2-way ANOVA and Dunnet’s multiple comparisons test comparing treatments to control (DMSO, 1%). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.