Figure 7.

Evaluation of cocultured donor and biosensor cells lacking Cx43 GJs. Activation of cocultures of Cx43-deficient donor and biosensor cells by the selective A_2AAR agonist CGS-21680. Forskolin (10 µM) was used as a positive control and DMSO (1%) served as a negative control. Data are normalized to the maximal effect of forskolin (100%). Data represent means ± SEM of three individual experiments performed in duplicates. Statistical significance calculated with repeated measures 2-way ANOVA and Dunnet’s multiple comparisons test comparing treatments to control (DMSO, 1%). ** p < 0.01, **** p < 0.0001.