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Calibration curve for the quantitation of pentadecanal. We added the indicated concentrations of the pentadecanal standard to the cell lysates. After the 5,5-dimethyl CHD derivative, we analyzed the SPE extract using C18 reverse-phase HPLC. The fluorescent peak area ratio of pentadecanal versus heptadecanal showed a linear curve with increasing pentadecanal concentrations. The data represent the mean ± SD of triplicate analyses.
