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. 2021 Feb 1;13(3):543. doi: 10.3390/cancers13030543

Figure 3.

Figure 3

miRNA-mediated activity of MC3324 on ERα expression. (A) Log2fold change of expression levels of different BC-correlated miRNAs from literature and found modulated in MCF-7 cells upon MC3324 treatment (25 µM, 24 h). Green and red dots represent down-regulated and up-regulated miRNAs, respectively. (B) Western blot showing expression levels of ERα in MCF-7 and MDA-MB-231 cells before and upon MC3324 treatment (25 µM, 24 h). (C) Expression of USP32, TBC1D1, and TBC1D9 after MC3324 induction. MCF-7 and MDA-MB-231 cells were treated with MC3324 for 6 and 24 h at 25 µM. The results of three independent experiments each with three replicates are represented as the mean ± SEM (standard error of the mean).* p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; n.s. for not significant variation.