Figure 5.

miR-181a-5p target validation. (A) miRNA relative expression levels determined by qPCR after transfection with synthetic mimic and inhibitor of miR-181a-5p and controls at a concentration of 100 nM for 48 h. (B) Relative expression levels of predicted target genes of miR-181a-5p determined by qPCR. RNU6B and GAPDH were used for data normalization for miRNA and gene expression. The results of three independent experiments each performed in triplicate are represented as the mean ± SD. ** p ≤ 0.01; *** p ≤ 0.001; n.s. for not significant variation