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. 2021 Feb 2;22(3):1469. doi: 10.3390/ijms22031469

Figure 5.

Figure 5

Expression of RD26 and HIS4 in the shoot apical meristems (SAMs) of SF-primed Arabidopsis plants. Expression of both genes was determined by RNA in situ hybridization. (a) Drought-induced expression of RD26 in the SAMs of SF-primed and unprimed plants. Note the moderate expression of RD26 in unprimed, drought-stressed plants at 36 DAG (days after germination; day 5 of drought) and the highly induced expression at 42 DAG (11 days of drought). (b) Expression of HIS4 (marker of the S phase of the cell cycle) in the SAM. (c) Number of HIS4-positive cells in the SAMs of SF-primed and unprimed plants. In (b,c), note the absence (NA) of HIS4-positive cells in unprimed drought-stressed plants at 42 DAG (i.e., after 11 days of drought), but the high number of HIS4-positive cells in SAMs of SF-primed plants, even after extended drought. Error bars indicate standard deviation (n = 3). Statistical significance was calculated using Student’s t-test (* p < 0.05; ** p < 0.01). Dr, drought stress; SF, Super Fifty.