Table 1.
Super Fifty and drought alter the expression of genes representing the activation of drought signaling pathways for stress tolerance. Values are trimmed means of M-values (TMMs) averaged across three biological replicates. Dr, drought stress; SF, Super Fifty.
| AGI Code | Gene Name | Description/Function | Expression Values (TMM) | |||
|---|---|---|---|---|---|---|
| Untreated (H2O+H2O) |
Drought-Stressed (H2O+Dr) | SF-Primed (SF+H2O) | SF-Primed+Drought-Stressed (SF+Dr) | |||
| Genes induced by SF priming in the absence of drought stress | ||||||
| AT2G29460 | GSTU4 | Glutathione S-transferase tau 4 | 1.49 | 605.19 | 20.11 | 23.76 |
| AT2G29350 | SAG13 | Senescence-associated gene 13 | 3.69 | 664.02 | 85.49 | 35.16 |
| AT5G40390 | RFS5, SIP1 | Raffinose synthase family protein | 1.08 | 92.61 | 8.01 | 25.74 |
| AT1G52690 | LEA7 | Late embryogenesis abundant protein | 2.42 | 6582.52 | 54.96 | 123.57 |
| AT5G06760 | LEA4-5, LEA46 | Late embryogenesis abundant 4–5 protein | 0.31 | 2817.88 | 35.40 | 300.14 |
| AT2G35980 | YLS9 | Yellow-leaf-specific gene 9 | 0.05 | 6.90 | 1.06 | 0.70 |
| AT2G42560 | LEA25 | Late embryogenesis abundant protein | 0.04 | 418.16 | 6.80 | 1.38 |
| AT3G02480 | ABR | Late embryogenesis abundant protein | 15.41 | 4582.45 | 105.24 | 138.61 |
| AT3G44300 | NIT2 | Nitrilase 2 | 0.84 | 416.61 | 101.37 | 55.04 |
| AT2G37180 | PIP2-3, RD28 | Aquaporin-like superfamily protein | 17.92 | 6.69 | 58.51 | 27.92 |
| AT2G37170 | PIP2B | Plasma membrane intrinsic protein 2 | 66.91 | 11.31 | 171.42 | 143.84 |
| AT4G12490 | AZI3 | Lipid-transfer protein | 0.04 | 0.64 | 6.88 | 2.73 |
| AT4G12480 | EARLI1 | Lipid-transfer protein (putative) | 0.70 | 0.45 | 8.00 | 1.93 |
| Genes involved in ABA signaling | ||||||
| AT4G03415 | PP2C52 | Protein phosphatase 2C family protein | 27.41 | 5.66 | 24.75 | 13.91 |
| AT4G26080 | ABI1 | Protein phosphatase 2C family protein | 21.23 | 557.80 | 57.47 | 311.62 |
| AT5G57050 | ABI2 | Protein phosphatase 2C family protein | 2.94 | 308.02 | 11.87 | 90.17 |
| AT2G29380 | HAI3 | Highly ABA-induced PP2C protein 3 | 0.07 | 73.94 | 0.53 | 0.23 |
| AT1G78290 | SNRK2.8, SRK2C | Protein kinase superfamily protein | 3.36 | 1.25 | 6.24 | 10.67 |
| AT4G34000 | ABF3 | Abscisic acid-responsive elements-binding factor 3 | 2.24 | 32.19 | 5.66 | 66.94 |
| AT5G47910 | RBOHD | Respiratory burst oxidase homologue D | 19.56 | 11.63 | 24.85 | 31.15 |
| AT5G53160 | PYL8, RCAR3 | Regulatory components of ABA receptor 3 | 194.42 | 21.54 | 228.40 | 87.45 |
| AT3G11410 | PPT2CA | Protein phosphatase 2CA | 70.86 | 1061.27 | 131.39 | 848.51 |
| AT4G25480 | DREB1A | Dehydration response element binding B1A | 0.04 | 1.99 | 0.20 | 6.42 |
| AT5G11590 | DREB3, TINY2 | Dehydration response element binding B3 | 1.70 | 0.63 | 1.50 | 14.27 |
| AT5G47640 | NF-YB2 | Nuclear factor Y, subunit B2 | 52.72 | 350.49 | 81.55 | 1474.77 |
| AT4G14540 | NF-YB3 | Nuclear factor Y, subunit B3 | 86.44 | 20.03 | 104.90 | 108.38 |
| AT5G06510 | NF-YA10 | Nuclear factor Y, subunit A10 | 2.36 | 6.64 | 2.64 | 11.46 |
| Genes linked to ERF53-dependent transcription | ||||||
| AT2G20880 | ERF53 | AP2/ERF transcription factor 53 | 0.15 | 1.23 | 4.82 | 6.85 |
| AT3G01650 | RGLG1 | RING domain ligase 1 | 4.16 | 60.77 | 4.46 | 4.13 |
| AT5G14420 | RGLG2 | RING domain ligase 2 | 11.96 | 36.40 | 12.06 | 10.88 |
| AT5G47220 | ERF2 | AP2/ERF transcription factor 2 | 11.75 | 6.85 | 16.90 | 29.06 |
| AT4G28140 | ERF54 | AP2/ERF transcription factor 54 | 0.83 | 6.98 | 13.39 | 23.40 |
| AT1G06160 | ERF94, ORA59 | Octadecanoid-responsive AP2/ERF transcription factor 59 | 2.58 | 0.10 | 4.82 | 4.95 |
| Genes involved in antioxidant ascorbate glutathione cycle and ROS homeostasis | ||||||
| AT4G09010 | APX4 | Ascorbate peroxidase 4 | 117.31 | 37.68 | 96.79 | 137.32 |
| AT1G77490 | APXT, TAPX | Thylakoidal ascorbate peroxidase | 43.36 | 9.34 | 48.15 | 71.70 |
| AT5G16710 | DHAR3 | Dehydroascorbate reductase 1 | 216.89 | 94.41 | 215.00 | 339.78 |
| AT2G25080 | GPX1 | Glutathione peroxidase 1 | 161.75 | 78.19 | 168.16 | 171.16 |
| AT3G55040 | GSTL2 | Glutathione transferase lambda 2 | 130.57 | 68.16 | 151.44 | 192.82 |
| AT1G03680 | ATHM1, THM1 | Thioredoxin M-type 1 | 517.98 | 248.21 | 601.57 | 842.87 |
| AT3G15360 | ATHM4, TRX-M4 | Thioredoxin M-type 4 | 354.88 | 254.22 | 394.58 | 596.43 |
| AT1G28480 | GRX480, GRXC9 | Thioredoxin superfamily protein | 0.61 | 2.20 | 1.26 | 7.46 |
| AT1G50320 | ATHX, THX | Thioredoxin X | 358.33 | 230.93 | 425.76 | 532.61 |
| AT3G11630 | BAS1 | Thioredoxin superfamily protein | 527.03 | 153.38 | 541.26 | 712.82 |
| AT3G26060 | ATPRX Q, PRXQ | Thioredoxin superfamily protein | 239.96 | 62.0 | 251.24 | 333.39 |
| AT4G03520 | ATHM2 | Thioredoxin superfamily protein | 513.22 | 324.57 | 578.12 | 760.03 |
| AT4G15660 | GRXS8 | Thioredoxin superfamily protein | 32.06 | 4.34 | 26.35 | 21.11 |
| Genes involved in cytokinin-mediated stomatal closure | ||||||
| AT3G49120 | PRX34 | Peroxidase 34 | 50.30 | 5938.87 | 258.46 | 381.85 |
| AT4G16110 | ARR2 | Response regulator 2 | 3.39 | 18.33 | 3.67 | 3.29 |
| AT3G25250 | OXI1 | Oxidative signal-Inducible 1 | 0.06 | 1.97 | 0.25 | 0.29 |
| AT5G51060 | RBOHC, RHD2 | Respiratory Burst Oxidase Homolog C | 0.06 | 2.51 | 0.16 | 0.04 |
| Genes involved in growth regulation and cell cycling | ||||||
| AT3G21870 | CYCP2;1 | Cyclin P2;1 | 3.57 | 0.95 | 8.87 | 16.75 |
| AT1G47210 | CYCA3;2 | Cyclin-dependent protein kinase 3;2 | 4.35 | 4.54 | 4.22 | 14.86 |
| AT2G28740 | HISTONE H4 | Histone H4 (HIS4) | 38.66 | 29.96 | 29.51 | 72.47 |
| AT4G27410 | RD26 | Responsive to desiccation 26 | 6.33 | 1086.04 | 37.74 | 313.78 |
| AT1G19350 | BES1 | BRI-EMS suppressor 1 | 131.59 | 27.67 | 148.71 | 135.43 |