Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 1;13(3):561. doi: 10.3390/cancers13030561

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Construction of the C promoter deletion (dCp) and revertant (rev) EBV mutant constructs using the EBV-bacterial artificial chromosome (BAC) system. (A) Schematic diagram of the EBV-BAC recombination. The Neo/St cassette was inserted first in the Cp. Neo/St cassette was replaced with a Cp sequence with deletion from 11,145 to 11,476 (332 bp) of KC207813.1. The Neo/St cassette was reinserted again and then replaced with the wild-type (WT) Cp sequence to construct the rev virus. (B,C) Recombinant EBV-BAC DNA was digested by BamHI and EcoRI, and the products were examined by agarose gel electrophoresis. Note that the digested product near 9.4 kbp was shortened in the dCp virus (indicated by arrowhead), reflecting the deletion.