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Construction of the latent membrane protein 2A-deficient (dLMP2A) and Cp/LMP2A double knockout (dCpdLMP2A) EBV mutants using the EBV-BAC system. (A) Schematic diagram of the EBV-BAC recombinant viruses. The first exons of the LMP2A gene of WT and dCp EBV constructs were removed to prepare dLMP2A and dCpdLMP2A, respectively. (B) Recombinant EBV-BAC DNA was digested by BamHI and EcoRI, and the products were examined by agarose gel electrophoresis.
