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. 2021 Feb 1;13(3):561. doi: 10.3390/cancers13030561

Figure 8.

Figure 8

Construction of the BHRF1 deficient (dBHRF1) and Cp/BHRF1 double knockout (dCPdBHRF1) EBV mutants using the EBV-BAC system. (A) Schematic diagram of the EBV-BAC recombinant viruses. Two adenine residues were inserted between nucleotide 194 and 195 of BHRF1 amino acids to make a stop codon (T194AA). (B,C) Recombinant EBV-BAC DNA was digested by BamHI and EcoRI, and the products were examined by agarose gel electrophoresis.