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. 2021 Feb 1;13(3):561. doi: 10.3390/cancers13030561

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Transformation assay of dBHRF1 and dCpdBHRF1 viruses. Recombinant viruses (WT, dBHRF1, dCp, and dCpdBHRF1) were produced from HEK293 cells. After titer normalization of the viruses, 10-fold serial dilutions were prepared and used to separately infect PBMCs. For each dilution, 8 wells were prepared. (A,B) Ratio of transformed wells with a multiplicity of infection (MOI) of 0.2 (A) and 0.02 (B) over time. (C,D) Transformation efficiency at 3 weeks post-infection. Two independent experiments were carried out using PBMCs from two healthy donors (exp. 1 and exp. 2).