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Kinetic parameter of Lxmdh wild-type and its mutants with methanol as a substrate.
| Enzyme |
kcat (min−1) |
KM (mM) |
kcat/KM (min−1.mM−1) (Relative Catalytic Efficiency) |
|---|---|---|---|
| Wild-type | 2.0 ± 0.2 | 1.311 ± 0.406 | 1.526 (1) |
| E396V | 1.2 ± 0.1 | 0.010 ± 0.003 | 120 (79) |
| E396V+K318N | 1.3 ± 0.1 | 0.233 ± 0.107 | 5.579 (4) |
| K318N | 1.6 ± 0.3 | 0.046 ± 0.072 | 34.78 (23) |
| K46E | 1.7 ± 0.2 | 0.372 ± 0.231 | 4.570 (3) |
