Figure 2.
Gluconeogenic activity of Pfk B. (A) Dependence of Pfk B activity on the concentration of fructose-1,6-bisphosphate (F16bP). The concentration of ADP was kept at 1 mM while the concentration of F16bP was varied between 0–50 mM. (B) Dependence of Pfk B activity on the concentration of nucleoside diphosphates (NDPs). The concentration of F16bP was kept at 5 mM while the concentration of NDP was varied between 0–20 mM. Error bars are standard deviations from three independent experiments. The data in panel (A,B) were fitted to Equations (1) and (4).