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. 2021 Feb 2;13(3):569. doi: 10.3390/cancers13030569

Figure 2.

Figure 2

AZD3965 induces minor changes in the monocarboxylate (MCT) repertoire of breast-associated malignant and nonmalignant cells when lactate is used as an oxidative fuel. (A–D) Cells were assayed in medium containing 10 mmol/L sodium L-lactate, no glucose, no glutamine and 1% FBS. (A) mRNA (left panel) and protein (middle and right panels) expression of MCT1, MCT2, MCT4 and CD147/basigin in T47D cells treated for 24 h ± 10 µM AZD3965 (n = 3–9 for RT-qPCR, n = 3 for WB). (B) As in A, but using MCF7 cells (n = 9 for RT-qPCR, n = 3–11 for WB). (C) As in A, but using MCF10A cells (n = 9–26 for RT-qPCR, n = 3–6 for WB). (D) As in A, but using BJ fibroblasts (n = 8–21 for RT-qPCR, n = 3–6 for WB). All data are shown as means ± SEM. * p < 0.05, ** p < 0.01, ns p > 0.05 compared to vehicle; by Student’s t test (A–D).