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. 2021 Feb 2;13(3):569. doi: 10.3390/cancers13030569

Figure 6.

Figure 6

AZD3965 is not cytotoxic for proliferative breast-associated cells when glucose and glutamine are available as metabolic fuels. (A) Density of T47D, MCF7, MCF10A cells and BJ fibroblasts over time when cultured in medium containing 25 mmol/L glucose with 10 mmol/L GlutaMAX, 10% FBS and without added lactate (white circles) or in medium containing 10 mmol/L sodium L-lactate, no glucose, no glutamine and 1% FBS (black circles) (n = 8–16). (B,C) Cell density was assayed in medium containing 25 mmol/L glucose with 10 mmol/L GlutaMAX, 10% FBS and without added lactate. (B) Cell density over time after a single treatment (arrow) with vehicle or different doses of AZD3965 (n = 8). (C) Cell density over time upon daily treatments (arrows) with vehicle or a given dose of AZD3965 (n = 7–8). All data are shown as means ± SEM. *** p < 0.005 comparing whole curves using two-way ANOVA (A). Analysis of cell proliferation (B,C) is presented in Table 2.