Figure 3.

NTP treatment reduced tumor volume and decreased CD47 expression, slightly, in vivo. (A) A schematic of the experimental design is provided. (B) B16F10 melanoma tumors were established in syngeneic B57BL/6 mice and treated directly with NTP for 5 consecutive days. Following treatment, tumors were resected immediately or 72 h post treatment and (C) stained for CD47 (red) and counterstained with DAPI (blue). All images were taken on the same day at 20× and batch processed for quantification to limit variations. (D) Tumor volumes were also reduced after treatment. (E) Quantification of CD47 was normalized to the untreated. Data here are represented as mean ± SEM and individual values are also shown (n = 3–11). Statistical significance of all treatment conditions was determined using the generalized linear mixed model with post hoc Dunnett’s test comparison to untreated (* p ≤ 0.05).