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. 2021 Feb 2;13(3):578. doi: 10.3390/cancers13030578

Figure 4.

Figure 4

Downstream targets of AKT3 are phosphorylated in NE-like LNCaPs. AKT3 regulates the activity of Forkhead box O3 (FOXO3A) and Glykogen synthase kinase 3 (GSK3α/β) by phosphorylation. (A) LNCaP cells were cultivated in androgen free medium for 14 days. Representative western blot analysis of FOXO3A protein level and phosphorylation on Ser253 reveals no significant changes in protein level in comparison to phosphorylation. (B) Densitometrical quantification of four independent experiments prove a 5-fold increased phosphorylation of FOXO3A using β-actin as housekeeping gene. (C) Representative western blot analysis of GSK3α/β protein level and phosphorylation on Ser21/Ser9 demonstrates no modification of protein level, but an induction of phosphorylation. (D) Densitometrical quantification of four independent experiments show 3-fold increased phosphorylation on serine residues Ser21/Ser9 using β-actin as housekeeping gene.