Figure 2.

DDM mask creation. The acquired COL7 image is firstly segmented and the DDM (a) is then created with a 5 × 5 search window, that is the minimal window size that allows discriminating single pixels based on their locality (b) Pixels semantics: (1) isolated red pixels, with LDI = 0; (2) green pixels, isolated, but not alone in their 5 × 5 neighborhood, with LDI ≥ 2; (3) either isolated or “end-point” purple pixels, with LDI = 1; (4) connected white pixels, with LDI ≥ 2. Scale bar: 5 µm.