(A) Two-dimensional thin-layer chromatogram of phospholipids from Legionella gormanii. PC—phosphatidylcholine, DMPE—phosphatidyl-N,N-dimethylethanolamine, PE—phosphatidylethanolamine, MMPE—phosphatidyl-N-monomethylethanolamine, PG—phosphatidylglycerol, and DPG—diphosphatidylglycerol (cardiolipin). Solvent system: (1) first dimension—chloroform/methanol/water (14:6:1, v/v/v) and (2) second dimension—chloroform/methanol/glacial acetic acid (13:5:2, v/v/v). (B) PC biosynthesis pathways in L. pneumophila. CDP, diacylglycerol; Pcs, phosphatidylcholine synthase; PmtA, phospholipid N-methyltransferase; SAM, S-adenosylmethionine; SAH, S-adenosylhomocysteine; and CM, cytoplasmic membrane.