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. 2021 Feb 2;22(3):1497. doi: 10.3390/ijms22031497

Figure 3.

Figure 3

Real-time PCR and ELISA measurements of hepcidin expression in lipopolysaccharide (LPS)- and lipoteichoic acid (LTA)-treated THP-1 cells. Real-time PCR was performed with SYBR Green protocol. β-actin was used as internal control for the normalization. The relative expression of untreated controls was regarded as 1. The mRNA expressions of the treated cells were compared to their appropriate controls (6 h and 24 h). Secreted hepcidin concentrations of the culture media were determined with a human hepcidin ELISA kit according to the instructions of the manufacturer. (A) mRNA expression levels of HAMP of THP-1 cells treated with LTA and LPS isolated from different sources. (B) Secreted hepcidin concentration of THP-1 cells treated with S. aureus LTA, E. coli LPS and P. aeruginosa LPS. The columns represent mean values and error bars represent standard deviation (SD) of three independent determinations (n = 3). Real-time PCR determinations and ELISA measurements were carried out in triplicate in each independent experiment. An asterisk * marks p < 0.05 compared to the controls. A cross † indicates p < 0.05 compared to LTA treatment and a double cross ‡ shows p < 0.05 compared to P. aeruginosa LPS treatment.