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Growth of subcutaneous MC38 tumors promotes cachexia. (A–D) Initial body weight (IBW) (A), tumor-free final body weight (FBW) (B), BW change (C) and carcass weights (D) of C57BL/6 male mice (8 weeks) subcutaneously injected with MC38 tumor cells (1.0 × 106 cells/mouse in sterile PBS: MC38) or an equal volume of vehicle (control) (n = 5). (E–H) Fat (E), liver (F), heart (G) and spleen (H) weights normalized to IBW. Data presented as mean ± s.d. ** p < 0.01, **** p < 0.0001 vs. control.
