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. 2021 Feb 2;22(3):1486. doi: 10.3390/ijms22031486

Figure 7.

Figure 7

MC38 tumor-derived factors induce atrophy of C2C12 myotubes. Co-culturing of C2C12 myotubes with MC38 tumor cells for 48 h was performed, followed by assessment of myotube diameter (n = 400) (A). Myotubes were stained with anti-MHC (B). Representative Western blotting and protein quantification (fold-change vs. control) for phosphorylated and total STAT3 (n = 3) (C). Follow-up experiments were performed co-culturing MC38 tumor cells and C2C12 myotubes with or without INCB018424 (400 nM). Quantification of myotube diameter (n = 400) (D). Myotubes were stained with anti-MHC (E). Representative Western blotting and protein quantification (fold-change vs. control) for phosphorylated and total STAT3 (n = 2) (F). Scale bars: 100 µm. Data presented as mean ± s.d. ** p < 0.01, *** p < 0.001, **** p < 0.001.