Figure 1.

Design of the comparative study cultivating HUVEC in parallel under nonadherent and adherent conditions for up to 48 h is shown for one experimental repeat with three HUVEC seedings (n = 3). The study was performed in the six well format with all together six HUVEC seedings for each sample in two independent experiments (n = 6 in sum). Samples were S-2h till S-48h for sedimented HUVEC on low attachment plates (LAP) for periods up to 48 h in step C, whereby S-0h was analysed directly after step B. Periods in suspension are indicated by the time code of the sample description (S-2h, S-4h, S-8h, S-24h and S-48h). HUVEC controls W-48h and T-48h were grown on TCP for 48 h during step C, whereby T-48h samples were additionally treated with rhTNF-α to induce HUVEC inflammatory activation and apoptosis (Cell culture flask picture modified from Servier Medical Art; Licence: CC BY 3.0; https://smart.servier.com/).