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. 2021 Feb 2;22(3):1493. doi: 10.3390/ijms22031493

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Decrease of HUVEC viability and high fraction of apoptotic/dead cells at nonadherent conditions over time. Time-depended distribution of nonadherent HUVEC viability status (viable = Annexin-V and DAPI negative, apoptotic = Annexin-V positive and DAPI negative, dead = Annexin-V and DAPI positive) compared to TCP grown control (W-48h) and with supplementation of rhTNF-α (T-48h) as positive control for artificial apoptosis induction. Data shown as arithmetic mean ± standard deviation; * p compared to growth control on TCP (W-48h); # p compared in between samples of nonadherent HUVEC; n = 6 from two independent experimental series with n = 3 HUVEC seedings each.