Table 4.
Primary and secondary antibodies used.
| Antibodies | Host | Dilution | Structures Identified by Antibodies | Source |
|---|---|---|---|---|
| ab59348 (polyclonal antibody) | Rabbit | 1:500 | BCL-2 | Abcam (UK) |
| sc-651 (monoclonal antibody) | Rabbit | 1:200 | iNOS | Santacruz Biotechnology (Santa Cruz, CA, USA) |
| A0150 (polyclonal antibody) | Rabbit | 1:100 | MMP-9 | DAKO (Gloustrup, Denmark) |
| M0823 (monoclonal antibody) | Mouse | 1:20 | CD31 (endothelial cells of blood vessels) | DAKO (Gloustrup, Denmark) |
| M0851 (monoclonal antibody) | Mouse | 1:40 | Actin (smooth muscle cells of blood vessels) | DAKO (Gloustrup, Denmark) |
| ab212369 (monoclonal antibody) |
Mouse | VEGFR1 | Abcam (UK) | |
| M0725 (monoclonal antibody) | Mouse | 1:50 | Vimentin (fibroblasts) |
DAKO (Gloustrup, Denmark) |
| M0876 (monoclonal antibody) | Mouse | 1:75 | CD68 (macrophages) | DAKO (Gloustrup, Denmark) |
| M7254 (monoclonal antibody) |
Mouse | 1:50 | CD3 (lymphocytes) | DAKO (Gloustrup, Denmark) |
| Rhodamine Goat AP124R | Mouse | 1:100 | Secondary antibody | MerckMillipore (Billerica, MA, USA) |
| Fluorescein Goat AP132F | Rabbit | 1:100 | Secondary antibody | MerckMillipore (Billerica, MA, USA) |