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. Author manuscript; available in PMC: 2021 Aug 19.
Published in final edited form as: J Am Chem Soc. 2020 Aug 4;142(33):14080–14089. doi: 10.1021/jacs.0c01064

Figure 1. Orthogonal bioluminescence imaging with engineered luciferases and chemically modified luciferins.

Figure 1.

(a) Luciferase-mediated oxidation of luciferin produces a photon of light. (b) Schematic of parallel engineering to generate orthogonal luciferase-luciferin pairs. (c) Two π-extended luciferins with red-shifted emission. The analogs were designed to be more flexible at the C2′-C2′′ junction. The conformation of PhOH-Luc is partially restricted via intramolecular hydrogen bonding.