Table 1.

HPLC instrumentation and parameters.

Analyte(s)	Vicine, convicine, divicine, and isouramil in FBPI	Vicine in NP	Levodopa	Tyramine
LC system	Agilent Model 1260 (Agilent Technologies, Wilmington, DE, USA)
Detector	Agilent G4212B diode array		Agilent G1321A fluorescence
Detection wavelengths	276 nm, 214 nm, 273 nm, 278 nm, 283 nm	276 nm, 214 nm	Ex = 280 nm Em = 310 nm	Ex = 275 nm Em = 303 nm
LC column	YMC-Pack ODS-AQa	Zorbax Eclipse Plus C18b	Zorbax Eclipse Plus C18b	YMC-Pack ODS-AQa
Temperature	25°C	15°C	20°C	15°C
Mobile phase A	0.05 M KH2PO4, pH 2.9		1000/25 (v/v) 0.05 M KH2PO4, pH 2.9/ACN	0.02 M KH2PO4, pH 2.9
Mobile phase B	200/800 (v/v) H2O/ACN
Flow rate	0.5 mL/min	0.4 mL/min	0.4 mL/min	0.5 mL/min
Injection	10 μL	10 μL	5 μL	10 μL
Elution program	0% B 0-20 min, 100% B 20-25 min, 0% B 25-45 min (end)	0% B 0-25 min, 100% B 25-30 min, 0% B 30-45 min (end)	0% B 0-25 min, 100% B 25-30 min, 0% B 30-50 min (end)	0% B 0-5 min, 0-10% B 5-25 min (linear), 100% B 25-30 min, 0% B 30-45 min (end)

^a4.6 × 250 mm, 5 μm, 120 Å; Waters Corporation, Milford, MA, USA. ^b4.6 × 250 mm, 5 μm; Agilent Technologies, Wilmington, DE, USA.